An oligonucleotide microarray for microRNA expression analysis based on labeling RNA with quantum dot and nanogold probe
نویسندگان
چکیده
منابع مشابه
An oligonucleotide microarray for microRNA expression analysis based on labeling RNA with quantum dot and nanogold probe
MicroRNAs (miRNAs) play important regulatory roles in animals and plants by targeting mRNAs for cleavage or translational repression. They have diverse expression patterns and might regulate various developmental and physiological processes. Profiling miRNA expression is very helpful for studying biological functions of miRNAs. We report a novel miRNA profiling microarray, in which miRNAs were ...
متن کاملEnhanced oligonucleotide microarray labeling and hybridization.
Benchmarks Microarrays are a powerful tool for functional studies at the molecular level (1,2). Depending on the experiment, users have a variety of commercial and homemade choices. Most commercially available microarrays come with protocols already optimized for repro-ducibility and sensitivity. However, despite continued reductions in the cost of microarrays, commercially available labeling k...
متن کاملQuantitative oligonucleotide microarray data analysis with an artificial standard probe strategy.
Quantitative data analysis is an important element in several applications of DNA microarray, including mRNA expression profiling and estimation of infectious doses for pathogens. Here, we introduce an artificial standard probe strategy for quantitative pathogen detection using an oligonucleotide chip as a model system. The standard capture probe sequence was artificially designed to prevent no...
متن کاملOligoArrayDb: pangenomic oligonucleotide microarray probe sets database
OligoArrayDb is a comprehensive database containing pangenomic oligonucleotide microarray probe sets designed for most of the sequenced genomes that are not covered by commercial catalog arrays. The availability of probe sequences, associated with custom microarray fabrication services offered by many companies and cores presents the unequalled possibility to perform microarray experiments on m...
متن کاملMicroarray oligonucleotide probe designer: a Web service
Correspondence: Michael e Zwick Department of human genetics, emory University School of Medicine, Whitehead Biomedical Research Building, Suite 301, Atlanta, gA 30322, USA Tel +1 404 727 9924 Fax +1 404 727 3949 email [email protected] Abstract: Methods of genomic selection that combine high-density oligonucleotide microarrays with next-generation DNA sequencing allow investigators to character...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2005
ISSN: 1362-4962
DOI: 10.1093/nar/gni019